pEP4 E02S EN2K

LOCUS       pEP4 E02S EN2K               16206 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    pEP4 E02S EN2K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16206)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 16206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16206
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4641..5803
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4872..5794
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    6847..7420
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6870..7422
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    8907..9041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9772..11561
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(11863..13788)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(14227..14245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14313..14417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14418..15278
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14636..14655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15449..16037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15938..15957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg
      601 ccggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 cgttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcangagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgaccaat
     4381 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     4441 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     4501 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     4561 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     4621 attattgact agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     4681 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     4741 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4801 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4861 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     4921 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
     4981 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
     5041 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5101 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5161 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
     5221 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5281 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5341 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5401 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
     5461 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5521 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5581 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5641 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5701 gtaattctcg ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5761 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca
     5821 attctgcagt cgagctcaag cttcgaacta gggccgcgac tctagtgatt catgagtgtg
     5881 gatccagctt gtccccaaag cttgccttgc tttgaagcat ccgactgtaa agaatcttca
     5941 cctatgcctg tgatttgtgg gcctgaagaa aactatccat ccttgcaaat gtcttctgct
     6001 gagatgcctc acacggagac tgtctctcct cttccttcct ccatggatct gcttattcag
     6061 gacagccctg attcttccac cagtcccaaa ggcaaacaac ccacttctgc agagaagagt
     6121 gtcgcaaaaa aggaagacaa ggtcccggtc aagaaacaga agaccagaac tgtgttctct
     6181 tccacccagc tgtgtgtact caatgataga tttcagagac agaaatacct cagcctccag
     6241 cagatgcaag aactctccaa catcctgaac ctcagctaca aacaggtgaa gacctggttc
     6301 cagaaccaga gaatgaaatc taagaggtgg cagaaaaaca actggccgaa gaatagcaat
     6361 ggtgtgacgc agaaggcctc agcacctacc taccccagcc tttactcttc ctaccaccag
     6421 ggatgcctgg tgaacccgac tgggaacctt ccaatgtgga gcaaccagac ctggaacaat
     6481 tcaacctgga gcaaccagac ccagaacatc cagtcctgga gcaaccactc ctggaacact
     6541 cagacctggt gcacccaatc ctggaacaat caggcctgga acagtccctt ctataactgt
     6601 ggagaggaat ctctgcagtc ctgcatgcag ttccagccaa attctcctgc cagtgacttg
     6661 gaggctgcct tggaagctgc tggggaaggc cttaatgtaa tacagcagac cactaggtat
     6721 tttagtactc cacaaaccat ggatttattc ctaaactact ccatgaacat gcaacctgaa
     6781 gacgtgtgaa gatgaatcga attcccgcgg ccgctttctc gaggccatcg atgtttaaac
     6841 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6901 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6961 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7021 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7081 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7141 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7201 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7261 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7321 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7381 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca ccgcgatcgc
     7441 gaattcgcga tcgccatggc tgtcagcgac gcgctgctcc catctttctc cacgttcgcg
     7501 tctggcccgg cgggaaggga gaagacactg cgtcaagcag gtgccccgaa taaccgctgg
     7561 cgggaggagc tctcccacat gaagcgactt cccccagtgc ttcccggccg cccctatgac
     7621 ctggcggcgg cgaccgtggc cacagacctg gagagcggcg gagccggtgc ggcttgcggc
     7681 ggtagcaacc tggcgcccct acctcggaga gagaccgagg agttcaacga tctcctggac
     7741 ctggacttta ttctctccaa ttcgctgacc catcctccgg agtcagtggc cgccaccgtg
     7801 tcctcgtcag cgtcagcctc ctcttcgtcg tcgccgtcga gcagcggccc tgccagcgcg
     7861 ccctccacct gcagcttcac ctatccgatc cgggccggga acgacccggg cgtggcgccg
     7921 ggcggcacgg gcggaggcct cctctatggc agggagtccg ctccccctcc gacggctccc
     7981 ttcaacctgg cggacatcaa cgacgtgagc ccctcgggcg gcttcatggc cgagctcctg
     8041 cggccagaat tggacccggt gtacattccg ccgcagcagc cgcagccgcc aggtggcggg
     8101 ctgatgggca agttcgtgct gaaggcgtcg ctgagcgccc ctggcagcga gtacggcagc
     8161 ccgtcggtca tcagcgtcag caaaggcagc cctgacggca gccacccggt ggtggtggcg
     8221 ccctacaacg gcgggccgcc gcgcacgtgc cccaagatca agcaggaggc ggtctcttcg
     8281 tgcacccact tgggcgctgg accccctctc agcaatggcc accggccggc tgcacacgac
     8341 ttccccctgg ggcggcagct ccccagcagg actaccccga ccctgggtct tgaggaagtg
     8401 ctgagcagca gggactgtca ccctgccctg ccgcttcctc ccggcttcca tccccacccg
     8461 gggcccaatt acccatcctt cctgcccgat cagatgcagc cgcaagtccc gccgctccat
     8521 taccaagagc tcatgccacc cggttcctgc atgccagagg agcccaagcc aaagagggga
     8581 agacgatcgt ggccccggaa aaggaccgcc acccacactt gtgattacgc gggctgcggc
     8641 aaaacctaca caaagagttc ccatctcaag gcacacctgc gaacccacac aggtgagaaa
     8701 ccttaccact gtgactggga cggctgtgga tggaaattcg cccgctcaga tgaactgacc
     8761 aggcactacc gtaaacacac ggggcaccgc ccgttccagt gccaaaaatg cgaccgagca
     8821 ttttccaggt cggaccacct cgccttacac atgaagaggc atttttaaat cccagacagt
     8881 ggatatgacc cgtttctagt tcgaaggatc cagacatgat aagatacatt gatgagtttg
     8941 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     9001 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     9061 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     9121 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     9181 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9241 gagcagacaa gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag
     9301 gtcgatcgac tctagctaga ggatcgatgc cccgccccgg acgaactaaa cctgactacg
     9361 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     9421 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     9481 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     9541 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     9601 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     9661 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     9721 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     9781 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     9841 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     9901 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     9961 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
    10021 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
    10081 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
    10141 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
    10201 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
    10261 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
    10321 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
    10381 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
    10441 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
    10501 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
    10561 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
    10621 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
    10681 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
    10741 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
    10801 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
    10861 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    10921 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
    10981 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
    11041 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
    11101 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
    11161 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
    11221 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
    11281 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
    11341 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    11401 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
    11461 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
    11521 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
    11581 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
    11641 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
    11701 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
    11761 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
    11821 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
    11881 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
    11941 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
    12001 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
    12061 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
    12121 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
    12181 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
    12241 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
    12301 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
    12361 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
    12421 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
    12481 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
    12541 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
    12601 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
    12661 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
    12721 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
    12781 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
    12841 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
    12901 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
    12961 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
    13021 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
    13081 tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
    13141 tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc
    13201 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc
    13261 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
    13321 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
    13381 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
    13441 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
    13501 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
    13561 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
    13621 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
    13681 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
    13741 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
    13801 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
    13861 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
    13921 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
    13981 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
    14041 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
    14101 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
    14161 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattaat tcttgaagac
    14221 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    14281 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    14341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    14401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    14461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    14521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    14581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    14641 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
    14701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    14761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    14821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    14881 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    14941 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    15001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    15061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    15121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    15181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    15241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    15301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    15361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    15421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    15481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    15541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    15601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    15661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    15721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    15781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    15841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    15901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    15961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    16021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    16081 ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
    16141 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
    16201 gcgtcg
//